Product Name	Fibrillarin
Chinese Name
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	H4C3
React Species	Human,Mouse,Rat
Applications	WB=1:500-2000,ICC/IF=1:50-200,Flow-Cyt=1:50-100 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG/Kappa
Purification	Affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	S-腺苷-L-甲硫氨酸依赖的甲基转移酶，具有使RNA和蛋白质甲基化的能力。通过催化前核糖体RNA中核糖部分的位点特异性2'-羟基甲基化参与前rRNA加工。位点特异性由与底物碱基配对的引导RNA提供。甲基化发生在与引导RNA碱基配对的序列的特征距离处。可能催化C/D-RNP复合物中U6-snRNAs的2'-O-甲基化。U6-snRNA 2'-O-甲基化是mRNA剪接保真度所必需的。还通过介导组蛋白H2A（H2AQ104me）的“Gln-105”甲基化而充当蛋白质甲基转移酶，这是一种损害FACT复合物结合的修饰，并特异性存在于35S核糖体DNA位点。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:3000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: HeLa, HepG2, 293T Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 37 kDa Observed MW: 37 kDa Cell line: U-2 OS Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Comment: Color green is the positive signal for SLM-60560R

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