Product Name	SATB1
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	H6D4
React Species	Human,Mouse,Rat
Applications	WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,ICC/IF=1:100-500,IF=1:50-200,Flow-Cyt=1:50-100（Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG
Purification	Affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	促成 Xist RNA 介导的 X 失活启动的关键沉默因子，发生在胚胎发生和淋巴瘤中。在富含 AT 的特殊序列（共有 SATB1 结合序列 (CSBS)，在核基质或支架相关区域）与 DNA 结合。认为识别双链 DNA 的糖磷酸结构。通过与 DNA 的基质附着区 (MAR) 结合并诱导局部染色质环重塑，以磷酸化和乙酰化状态依赖性方式控制核和病毒基因表达的转录抑制因子。作为几种染色质重塑酶（例如 MHC-I 位点的 PML）的停靠位点，还可以直接向启动子和增强子募集辅抑制因子 (HDAC) 或辅激活因子 (HAT)。调节对来自胸腺细胞的免疫 T 细胞 CD8SP 的成熟至关重要的基因。需要转换胎儿珠蛋白种类，以及在红Cell differentiation过程中调节 β 和 γ 珠蛋白基因。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Jurkat, 2: THP-1 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 86 kDa Observed MW: 100 kDa Tissue: Human tonsil Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60618R Cell line: Jurkat Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Comment: Color green is the positive signal for SLM-60618R Cell line: Jurkat Fixative: 4% Paraformaldehyde Permeabilization: 90% Methanol Primary ab dilution: 1:100 Secondary ab: Goat anti Rabbit IgG Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for SLM-60618R

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