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Product Name IRDye® 800CW Goat Anti-Rabbit IgG H&L Chinese Name IRDye 800cw羊抗兔IgG (H+L)(进口分装) Alias IRDye® 800CW Goat anti-Rabbit IgG H&L; Immunoglobulin G; 山羊抗兔IgG H&L (IRDye® 800CW)预吸附二抗; ®IRDye 800cw 羊抗兔IgG (H+L); Specific References (9) | SL40295G-IRDye8 has been referenced in 9 publications.Immunogen Species Goat Clonality Polyclonal React Species Human, Mouse, Rat, Rabbit, Applications WB=1:10000-30000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Form Liquid Concentration 1.0 mg/ml immunogen Native Rabbit IgG Lsotype IgG1 Purification affinity purified by Protein G Buffer Solution 10 mM TBS (pH=7.4) with 1% BSA, 0.01% Sodium Azide and 50% glycerol. Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Product Detail IRDye800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L), Highly Cross Adsorbed
Producer: Li-Cor(USA)
Product Number: 926-32211
Fluorophore: IRDye 800CW(MW: 1166)
Excitation Wavelength: 778nm(in PBS)
Emission Wacelegth: 795nm(in PBS)
Fluorophore/Protein Ratio: 1.7 moles IRDye 800CW: 1mole IgG
IRDye 800CW antibodies can be used for a variety of applications, including Western blotting, In-Cell Western assays, In-Gel Westerns, and many others.
Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of rabbit IgG as well as the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western applications.
Product Picture Sample:
Lane 1,4,7: Mouse Testis tissue lysates
Lane 2,5,8: Rat Testis tissue lysates
Lane 3,6,9: Human MCF-7 cell lysates
Primary: Anti-beta-Actin (SL0061R) at 1/1:2000 dilution
Secondary: IRDye800CW Goat anti-Rabbit IgG (SL40295G-IRDye8) at 1:50000~200000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
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