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Product Name AffiniPure Goat Anti-Rabbit IgG H&L / HRP Chinese Name 辣根过氧化物酶标记亲和纯化羊抗兔IgG (H+L)(进口分装) Alias AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot)/HRP;
AffiniPure Goat Anti-Rabbit IgG H&L/HRP;
AffiniPure Goat Anti-Rabbit IgG H&L(HRP);
HRP AffiniPure Goat Anti-Rabbit IgG (H+L);
Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) HRP标记山羊抗兔IgG (H+L);
辣根过氧化物酶标记羊抗兔IgG (H+L)(进口分装);
Specific References (59) | SL40295G-HRP has been referenced in 59 publications.Immunogen Species Goat Clonality Polyclonal React Species Rabbit, Applications WB=1:5000-20000 ELISA=1:5000-20000 IHC-P=1:3000-5000 IHC-F=1:3000-5000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Form Liquid Concentration 1.0 mg/ml immunogen Native Rabbit IgG Lsotype IgG Purification affinity purified by Protein G Buffer Solution 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Product Detail HRP Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L), Highly Cross Adsorbed
HRP antibodies can be used for a variety of applications, including ELISA, Western blotting, In-Cell Western assays, In-Gel Westerns, and many others.
Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of rabbit IgG as well as the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and ELISA applications.
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (SL5960R) at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (SL40295G-HRP ) at 1/200 dilution and DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (SL5960R) at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (SL40295G-HRP ) at 1/200 dilution and DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (SL5960R) at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (SL40295G-HRP ) at 1/200 dilution and DAB staining.
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