DESCRIPTION

Used for the quantitative determination of Anti-MPXV A29L Human IgG concentration in serum and plasma.

PRINCIPLE OF THE ASSAY

This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant MPXV A29L antigen has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-MPXV A29L Human IgG present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgG antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-MPXV A29L Human IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.

PRECISION

Intra-Assay Precision (Precision within an assay): <10% Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision. Inter-Assay Precision (Precision between assays): <15% Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

Sample		1	2	3	1	2	3
n		16	16	16	24	24	24
Mean (ng/mL)		8.5	2.3	1.2	9.2	2.3	1.3
Standard deviation		0.5	0.1	0.1	0.3	0.2	0.2
CV \(%\)		5.6	5.6	6.4	3.4	6.9	12.4

RECOVERY

The recovery of Anti-MPXV A29L Human IgG spiked to three different levels in human serum samples throughout the range of the assay was evaluated.

Sample	Dilution factor	Average (%)	Range (%)
Human serum (n=3)	1:1000	93	80-109
	1:2000	95	82-110

LINEARITY

To assess the linearity of the assay, human serum samples were spiked with high concentrations of Anti-MPXV A29L Human IgG and diluted with the Standard Diluent to produce samples with values within the dynamic range of the assay.

Sample	Dilution factor	Average (%)	Range (%)
Human serum (n=5)	1:2	97	93-104
	1:4	101	98-106
	1:8	104	99-113
	1:16	101	85-116

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)