DESCRIPTION

MSR1, also known as scavenger receptor-A (SR-A) or cluster of differentiation 204 (CD204), was first described in 1979 by Brown and Goldstein. MSR1 is highly expressed in the lung, arteries, and adipose tissue.MSR1 is predominantly expressed in various macrophage cell types and is mainly altered in macrophage-associated physiological and pathological processes, including atherosclerosis, Alzheimer's disease, host defence, and cancer.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	8.18	0.19	2.32
2	8	2.41	0.07	2.90
3	8	1.33	0.05	3.76

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	8.44	0.34	4.03
2	16	2.43	0.06	2.47
3	16	1.37	0.08	5.84

RECOVERY

The recovery of human CD204 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:4	123	117-131
	1:8	90	88-94
Cell lysate	1:4	98	97-99
	1:8	99	96-103

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Human serum	Cell lysate
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	100	104
	Range (%)	99-101	99-109
1:8	Average% of Expected	102	99
	Range (%)	99-106	81-118
1:16	Average% of Expected	101	113
	Range (%)	98-106	101-126

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human CD204 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
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• Price: USD, per kit (48T / 96T options available)