DESCRIPTION

CP110 is a centrosomal protein with a molecular weight of approximately 110 kDa, encoded by the CCP110 gene. It acts as a substrate for cell cycle protein-dependent kinase (CDK) during the cell cycle and plays a key role in centrosome replication and maturation. CP110 regulates cellular function by limiting centriole elongation and cilia formation. In addition, it cooperates with CEP97 as a negative regulator of cilia formation, preventing cilia formation by covering the distal end of the maternal centriole. However, in the in vivo setting, CP110 is also involved in distal appendage assembly and ciliary vesicle docking during cilia formation, thereby promoting cilia formation.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	9.65	0.27	2.77
2	8	2.36	0.14	5.96
3	8	1.25	0.06	4.44

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	9.57	0.32	3.31
2	16	2.35	0.10	4.37
3	16	1.22	0.06	5.15

RECOVERY

The recovery of human CP110 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:16	106	102-108

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:2. )

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	88
	Range (%)	81-95
1:8	Average% of Expected	77
	Range (%)	74-80
1:16	Average% of Expected	80
	Range (%)	78-82

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human CP110 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)