DESCRIPTION

Cyclooxygenase 1 (COX-1), also known as prostaglandin-endoperoxide synthase 1 (PTGS1), is an enzyme that in humans is encoded by the PTGS1 gene. COX-1 regulates angiogenesis in endothelial cells and also involved in cell signaling and maintaining tissue homeostasis. There is no COX-1 expression in fetal samples (prostate, seminal vesicles, or ejaculatory ducts), and only minimal expression in adult tissues.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	25.66	1.71	6.66
2	8	6.19	0.32	5.10
3	8	3.06	0.08	2.53

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	25.70	2.16	8.41
2	16	5.79	0.53	9.10
3	16	3.06	0.11	3.53

RECOVERY

The recovery of human COX-1/Cyclooxygenase-1 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:32	126	125-128

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:4.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	98
	Range (%)	93-103
1:8	Average% of Expected	97
	Range (%)	92-101
1:16	Average% of Expected	94
	Range (%)	85-103

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human COX-1/Cyclooxygenase-1 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
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