Product Name	Recombinant Rabbit Anti-POLR2A Monoclonal Antibody
Cat No.	SLA1158M
Chinese Name	磷酸化RNA聚合酶II CTD抗体重组兔单抗
Alias	NEDHIB; POLR2; POLRA; RPB1; RPBh1; RPO2; RPOL2; RpIILS; hRPB220; hsRPB1; 220kDa; Rpo2-1; RPB1_HUMAN; POLR2A; RNA polymerase II subunit B1; 3'-5' exoribonuclease; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1; 2.7.7.6; RPB1_MOUSE; Rpii215; RNA polymerase II subunit A; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; POLR2A | RNA polymerase II CTD repeat YSPTSPS (phospho-S2); p-RNA polymerase II CTD repeat YSPTSPS; phospho-RNA polymerase II CTD repeat YSPTSPS; p-POLR2A; POLR2A | RNA polymerase II CTD repeat YSPTSPS (phospho-Ser2)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	9F2
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) ChIP (6μg/IP) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human RNA polymerase II CTD around the phosphorylation site of Ser2
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	217 kDa
Subcellular Location	Nucleus
Gene ID	5430
Swiss-Prot	P24928
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.