Product Name	Recombinant Rabbit Anti-TG Monoclonal Antibody
Cat No.	SLA3109M
Chinese Name	甲状腺球蛋白重组兔单抗
Alias	AITD3; TGN; cog; THYG_HUMAN; TG; THYG_MOUSE; THYG_RAT; thyroglobulin
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	6C9
React Species	Human,Mouse,Rat
Applications	IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Flow-Cyt (1:50-100) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Thyroglobulin
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	305 kDa
Observed MW.
Subcellular Location	Secreted
Gene ID	7038
Swiss-Prot	P01266
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Thyroglobulin is the glycoprotein precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). Thyroglobulin is obtained from the thyroid gland and exhibits the general properties of the globulins. The human thyroglobulin (hTG) is a high molecular weight glycoprotein (605 kDa) found in the thyroid follicular cells. It plays a central role in the uptake, incorporation, and regulated biosynthesis of thyroid hormones, T4 and T3. Thyroid disorders are, in large part, due to autoimmune origin, and anti thyroglobulin autoantibodies were the first factor to be discovered. Anti hTG is found in all thyroid autoimmune diseases (Hashimoto's thyroiditis, Graves' diseases), with the highest level observed in Hashimoto's thyroiditis. Anti hTG is also characteristic of thyroid cancer, and its determination can be used for the follow up of cancer patients.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Rat Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TG Monoclonal Antibody, Unconjugated (SLA3109M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TG Monoclonal Antibody, Unconjugated (SLA3109M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Thyroid Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TG Monoclonal Antibody, Unconjugated (SLA3109M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. The TT (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Thyroglobulin antibody (SLA3109M,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (SL60295G-BF488): 1 μg/test. Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed
Figure 5. 4% Paraformaldehyde-fixed TT (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Thyroglobulin) monoclonal Antibody, unconjugated (SLA3109M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.