Product Name	Recombinant Rabbit Anti-RPS6KA1 Monoclonal Antibody
Cat No.	SLA3071M
Chinese Name	丝氨酸/苏氨酸激酶p90RSK蛋白重组兔单抗
Alias	KS6A1_MOUSE; Rps6ka1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1 (p90-RSK 1 | p90RSK1 | p90S6K); MAP kinase-activated protein kinase 1a (MAPK-activated protein kinase 1a | MAPKAP kinase 1a | MAPKAPK-1a); Ribosomal S6 kinase 1 (RSK-1); 2.7.11.1; Mapkapk1a; Rsk1; KS6A1_RAT; HU-1; MAPKAPK1; RSK; p90Rsk; ribosomal protein S6 kinase A1; ribosomal protein S6 kinase, 90kD, polypeptide 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	5H2
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human p90 S6K alpha 1
Purification	affinity purified by Protein A
Predicted MW.	83 kDa
Observed MW.	83 kDa
Subcellular Location	Cytoplasm
Gene ID	6195
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells. Transiently dissociates following mitogenic stimulation. Interacts with ETV1/ER81 and FGFR1.Interacts with Kaposi's sarcoma-associated herpesvirus/HHV-8 protein ORF45; this interaction allows RPS6KA1 activation.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with RPS6KA1 monoclonal antibody, unconjugated (SLA3071M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6KA1 Monoclonal Antibody, Unconjugated (SLA3071M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6KA1 Monoclonal Antibody, Unconjugated (SLA3071M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6KA1 Monoclonal Antibody, Unconjugated (SLA3071M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6KA1 Monoclonal Antibody, Unconjugated (SLA3071M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.