Product Name	Recombinant Rabbit Anti-Casp7 Monoclonal Antibody
Cat No.	SLA3114M
Chinese Name	半胱胺酸蛋白酶-7重组兔单抗
Alias	CASP-7; CMH-1; ICE-LAP3; LICE2; MCH3; ICE-IAP3; caspase-7; mCASP-7; CASP7_MESAU; CASP7; Apoptotic protease Mch-3; ICE-like apoptotic protease 3 (ICE-LAP3); SREBP cleavage activity 2 (SCA-2); 3.4.22.60; CASP7_HUMAN; CASP7_MOUSE; Cysteine protease LICE2; caspase 7; caspase 7, apoptosis-related cysteine protease; caspase 7, apoptosis-related cysteine peptidase
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	7G9
React Species	Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from mouse Caspase 7
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	34 kDa
Observed MW.	36 kDa
Subcellular Location	Nucleus
Gene ID	12369
Swiss-Prot	P97864
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of this caspase is cleaved by caspase3 and 10. It is activated upon cell death stimuli and induces apoptosis. Alternative splicing results in four transcript variants, encoding three distinct isoforms. [provided by RefSeq].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Caspase-7 monoclonal antibody, unconjugated (SLA3114M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase 7 Monoclonal Antibody, Unconjugated (SLM-60564R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase 7 Monoclonal Antibody, Unconjugated (SLM-60564R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase 7 Monoclonal Antibody, Unconjugated (SLM-60564R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase 7 Monoclonal Antibody, Unconjugated (SLM-60564R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

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