Product Name	Recombinant Rabbit Anti-CYB5R3 Monoclonal Antibody
Cat No.	SLA2518M
Chinese Name	细胞色素b5还原酶3重组兔单抗
Alias	B5R; DIA1; Nadhcb5; RNNADHCB5; NB5R3_HUMAN; CYB5R3; Cytochrome b5 reductase; Diaphorase-1; 1.6.2.2; NB5R3_RAT; cytochrome b5 reductase 3; diaphorase (NADH) (cytochrome b-5 reductase); NADH-cytochrome b5 reductase 3
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Cytochrome b5 reductase
Purification	affinity purified by Protein A
Predicted MW.	34 kDa
Observed MW.	34 kDa
Gene ID	1727
Swiss-Prot	P00387
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with CYB5R3 monoclonal antibody, unconjugated (SLA2518M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYB5R3 Monoclonal Antibody, Unconjugated (SLA2518M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYB5R3 Monoclonal Antibody, Unconjugated (SLA2518M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYB5R3 Monoclonal Antibody, Unconjugated (SLA2518M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYB5R3 Monoclonal Antibody, Unconjugated (SLA2518M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.