Product Name	Recombinant Rabbit Anti-BLNK Monoclonal Antibody
Cat No.	SLA2517M
Chinese Name	B淋巴细胞连接蛋白重组兔单抗
Alias	AGM4; BASH; BLNK-S; LY57; SLP-65; SLP65; bca; Ly-57; Lyw-57; BLNK_HUMAN; BLNK; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; Cytoplasmic adapter protein; Src homology 2 domain-containing leukocyte protein of 65 kDa (SLP-65); BLNK_MOUSE; Lymphocyte antigen 57; BLNK_RAT; B cell linker; B-cell activation; Src homology [SH2] domain-containing leukocyte protein of 65 kD; B cell adaptor containing SH2 domain
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	1A12
React Species	Human,Mouse,Rat
Applications	IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Flow-Cyt (1:50-100) ICC/IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human BLNK
Purification	affinity purified by Protein A
Predicted MW.	50 kDa
Observed MW.	70 kDa
Subcellular Location	Cytoplasm
Gene ID	29760
Swiss-Prot	Q8WV28
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BLNK Monoclonal Antibody, Unconjugated (SLA2517M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BLNK Monoclonal Antibody, Unconjugated (SLA2517M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BLNK Monoclonal Antibody, Unconjugated (SLA2517M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. The Raji (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-BLNK antibody (SL62608R,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed
Figure 5. 4% Paraformaldehyde-fixed Raji (H) cell; Antibody incubation with (BLNK) monoclonal Antibody, unconjugated (SLA2517M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.