35.1 Cell-Specific Culture Medium - Basic Information

Culture Medium	90% RPMI-1640 + 10% FBS + 0.05 mM β-Mercaptoethanol + 2 mM L-Glutamine
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear and transparent, pale yellow
Procedure	① Preparation: UV sterilize pipettes, 50 mL centrifuge tubes, 500 mL serum bottles, and the clean bench for 20 minutes; pre-warm the basal medium in a 37°C water bath. ② Formulation: Flame-sterilize the mouths of the basal medium bottle and serum bottle; aseptically pour 50 mL of serum, 0.05 mM β-mercaptoethanol, and 2 mM glutamine into 450 mL of basal medium. ③ Sealing: Seal the culture medium bottle mouth with sealing film, attach the corresponding product label, and place the medium back in a 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and UV sterilize for 30 minutes.