MDA-MB-435S Cell Specialized Medium - Basic Information

Medium	90% L-15 + 10% FBS + 0.01mg/ml Insulin
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear and transparent, pale red
Procedure	① Preparation: UV-irradiate the pipette, 50mL centrifuge tubes, 500mL serum bottle, and clean bench for 20 minutes; pre-warm the basal medium in a 37°C constant temperature water bath; ② Preparation: Flame-sterilize the mouths of the basal medium bottle and serum bottle, then aseptically pour 50mL of serum and 0.01mg/mL insulin into 450mL of basal medium; ③ Sealing: Seal the culture medium bottle mouth with parafilm, attach the corresponding product label, and store the culture medium back in the 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and UV-irradiate for 30 minutes;