KMB-17 Cell-Specific Culture Medium - Basic Information

Culture Medium	89% DMEM-H + 10% FBS + 1% NEAA
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear and transparent, red in color
Procedure	① Preparation: Sterilize the pipette gun, 50mL centrifuge tube, 100mL serum bottle, and clean bench with UV light for 20 minutes; pre-warm the basal medium in a 37°C constant-temperature water bath; ② Formulation: Sterilize the mouths of the basal medium bottle and serum bottle with flame, and aseptically pour 10% FBS and 1% NEAA into 89% DMEM-H while suspended in air; ③ Sealing: Seal the mouth of the culture medium bottle with parafilm, attach the corresponding product label, and place the culture medium back into the 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and irradiate with UV for 30 minutes;