EMEM Complete Medium (with 2% FBS) - Basic Information

Medium	98% EMEM + 2% FBS
Storage Condition	Store at 2-8°C, valid for one year
Appearance	Pale red, clear and transparent
Procedure	① Prepare: Place the pipette, 50mL centrifuge tubes, 100mL serum bottles, and UV-sterilize the clean bench for 20 minutes; pre-warm the basal medium in a 37°C constant temperature water bath; ② Mix: Flame-sterilize the openings of the basal medium bottle and serum bottle, then aseptically pour 10mL of serum into 490mL of basal medium; ③ Seal: Seal the medium bottle with sealing film, attach the corresponding product label, and return the medium to a 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and UV-sterilize for 30 minutes.