Fc3Tg Specialized Medium - Basic Information

Medium	88% EMEM + 1% NEAA + 1% L-Glutamine + 10% FBS
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear and transparent, light red
Procedure	① Preparation: UV irradiate the pipette, 50mL centrifuge tube, 500mL serum bottle, and clean bench for 20 minutes; pre-warm the basal medium in a 37°C water bath; ② Formulation: Flame-sterilize the mouths of the basal medium bottle and serum bottle, then aseptically add 1% NEAA, 1% L-Glutamine, and 10% FBS into the 88% EMEM basal medium; ③ Sealing: Seal the culture medium bottle mouth with sealing film, attach the corresponding product label, and return the culture medium to the 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and UV irradiate for 30 minutes;