TCCSUP Cell-Specific Culture Medium - Basic Information

Culture Medium	87% EMEM + 10% FBS + 1% Glutamine + 1% Sodium Pyruvate + 1% NEAA
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear and transparent, light red
Procedure	① Preparation: Expose pipettes, 50 mL centrifuge tubes, 100 mL serum bottles, and the clean bench to UV light for 20 minutes; pre-warm the basal medium in a 37°C water bath. ② Preparation: Flame-sterilize the openings of the basal medium bottle and serum bottle, then pour 50 mL of serum into 435 mL of basal medium while suspended; add 1% glutamine, 1% sodium pyruvate, and 1% NEAA. ③ Sealing: Seal the culture medium bottle with sealing film, attach the corresponding product label, and place the medium back in a 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and UV sterilize for 30 minutes.