KMH-2 Cell-Specific Culture Medium - Basic Information

Culture Medium	45% DMEM-H + 45% RPMI-1640 + 10% FBS
Storage Conditions	Store at 2-8°C, protected from light, valid for one month
Appearance	Light red, clear and transparent
Operating Steps	① Preparation: UV-irradiate pipettes, 50mL centrifuge tubes, 500mL serum bottles, and the clean bench for 20 minutes; pre-warm the basal medium in a 37°C constant-temperature water bath; ② Preparation: Flame-sterilize the mouth of the basal medium bottle and serum bottle, pour 50mL of serum into 450mL of basal medium prepared by mixing RPMI-1640 and DMEM-H at a 1:1 ratio; ③ Sealing: Seal the culture medium bottle mouth with parafilm, attach the corresponding product label, and return the culture medium to a 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and UV-irradiate for 30 minutes;