Product Name	Recombinant Rabbit Anti-SMCHD1 Monoclonal Antibody
Cat No.	SLA2164M
Chinese Name	SMCHD1蛋白重组兔单抗
Alias	SMHD1_HUMAN; SMCHD1; SMC hinge domain-containing protein 1; 3.6.1.-; KIAA0650; SMHD1_MOUSE; structural maintenance of chromosomes flexible hinge domain containing 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	8A7
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human SMCHD1
Purification	affinity purified by Protein A
Predicted MW.	226 kDa
Observed MW.	220 kDa
Subcellular Location	N/A
Gene ID	23347
Swiss-Prot	A6NHR9
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Non-canonical member of the structural maintenance of chromosomes (SMC) protein family that plays a key role in epigenetic silencing by regulating chromatin architecture.Promotes heterochromatin formation in both autosomes and chromosome X, probably by mediating the merge of chromatin compartments.Plays a key role in chromosome X inactivation in females by promoting the spreading of heterochromatin.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with SMCHD1 monoclonal antibody, unconjugated (SLA2164M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMCHD1 Monoclonal Antibody, Unconjugated (SLA2164M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMCHD1 Monoclonal Antibody, Unconjugated (SLA2164M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMCHD1 Monoclonal Antibody, Unconjugated (SLA2164M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMCHD1 Monoclonal Antibody, Unconjugated (SLA2164M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (Red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.