Product Name	Recombinant Rabbit Anti-RAD23A Monoclonal Antibody
Cat No.	SLA2174M
Chinese Name	紫外切除修复蛋白RAD23A重组兔单抗
Alias	RD23A_HUMAN; RAD23A; HR23A; hHR23A; RD23A_MOUSE; mHR23A; RAD23 nucleotide excision repair protein A; RAD23 homolog A, nucleotide excision repair protein; RAD23 (S. cerevisiae) homolog A; RAD23 homolog A (S. cerevisiae); RAD23, yeast homolog, A
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	9D1
React Species	Human
Applications	WB (1:500-2000) Flow-Cyt (1:50-100) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human RAD23A
Purification	affinity purified by Protein A
Predicted MW.	40 kDa
Observed MW.	52 kDa
Subcellular Location	N/A
Gene ID	5886
Swiss-Prot	P54725
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (RAD23A) monoclonal Antibody, unconjugated (SLA2174M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, then stained with Phalloidin/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei	Figure 2. 4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (RAD23A) monoclonal Antibody, unconjugated (SLA2174M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min; DAPI (blue, C02-04002) was used to stain the cell nuclei
Figure 3. 25 ug total protein per lane of various lysates (see on figure) probed with RAD23A monoclonal antibody, unconjugated (SLA2174M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 4. The Jurkat (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-RAD23A antibody (SLA2174M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed
Figure 5. 4% Paraformaldehyde-fixed Jurkat (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (RAD23A) monoclonal Antibody, unconjugated (SLA2174M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

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