Product Name	Recombinant Rabbit Anti-MYL9 Monoclonal Antibody
Cat No.	SLA2115M
Chinese Name	肌球蛋白调节多肽9重组兔单抗
Alias	LC20; MLC-2C; MLC2; MMIHS4; MRLC1; MYRL2; MLC20; Mylc2c; RLC-C; MYL9_HUMAN; MYL9; 20 kDa myosin light chain (LC20); Myosin RLC; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; MYL9_MOUSE; MYL9_RAT; myosin light chain 9; myosin, light polypeptide 9, regulatory; myosin, light chain 9, regulatory; myosin regulatory light chain 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	8H5
React Species	Human,Mouse
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Flow-Cyt (1:50-100) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Myosin light chain 9
Purification	affinity purified by Protein A
Predicted MW.	20 kDa
Observed MW.	18 kDa
Subcellular Location	N/A
Gene ID	10398
Swiss-Prot	P24844
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with MYL9 monoclonal antibody, unconjugated (SLA2115M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MYL9 Monoclonal Antibody, Unconjugated (SLA2115M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MYL9 Monoclonal Antibody, Unconjugated (SLA2115M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MYL9 Monoclonal Antibody, Unconjugated (SLA2115M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-MYL9 antibody (SLA2115M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.