Product Name	Recombinant Rabbit Anti-FBP1 Monoclonal Antibody
Cat No.	SLA1998M
Chinese Name	果糖1，6-二磷酸酯酶重组兔单抗
Alias	FBP; Fbp-2; Fbp2; Fbp3; Fdp; F16P1_HUMAN; FBP1; FBPase 1; D-fructose-1,6-bisphosphate 1-phosphohydrolase 1; Liver FBPase; 3.1.3.11; F16P1_MOUSE; Fructose-1,6-bisphosphatase isozyme 3 (FBPase 3); F16P1_RAT; fructose-bisphosphatase 1; fructose-1,6-bisphosphatase 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	5E8
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human FBPase 1
Purification	affinity purified by Protein A
Predicted MW.	37 kDa
Observed MW.	38 kDa
Gene ID	2203
Swiss-Prot	P09467
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with FBP1 monoclonal antibody, unconjugated (SLA1998M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FBP1 Monoclonal Antibody, Unconjugated (SLA1998M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FBP1 Monoclonal Antibody, Unconjugated (SLA1998M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FBP1 Monoclonal Antibody, Unconjugated (SLA1998M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FBP1 Monoclonal Antibody, Unconjugated (SLA1998M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.