Product Name	Recombinant Rabbit Anti-VAMP1 Monoclonal Antibody
Cat No.	SLA1965M
Chinese Name	囊泡相关膜蛋白1重组兔单抗
Alias	CMS25; SAX1; SPAX1; SYB1; VAMP-1; Syb-1; lew; VAMP1_HUMAN; VAMP1; Synaptobrevin-1; VAMP1_MOUSE; VAMP1_RAT; vesicle associated membrane protein 1; synaptobrevin 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	3A6
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human VAMP1
Purification	affinity purified by Protein A
Predicted MW.	13 kDa
Observed MW.	16 kDa
Gene ID	6843
Swiss-Prot	P23763
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with VAMP1 monoclonal antibody, unconjugated (SLA1965M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with VAMP1 Monoclonal Antibody, Unconjugated (SLA1965M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with VAMP1 Monoclonal Antibody, Unconjugated (SLA1965M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with VAMP1 Monoclonal Antibody, Unconjugated (SLA1965M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with VAMP1 Monoclonal Antibody, Unconjugated (SLA1965M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.