Product Name	Recombinant Rabbit Anti-GNB2 Monoclonal Antibody
Cat No.	SLA2036M
Chinese Name	G蛋白β2重组兔单抗/鸟嘌呤核苷酸结合蛋白β亚基2重组兔单抗
Alias	HG2C1; NEDHYDF; SSS4; Gnb-2; GBB2_HUMAN; GNB2; G protein subunit beta-2; Transducin beta chain 2; GBB2_MOUSE; GBB2_RAT; G protein subunit beta 2; guanine nucleotide binding protein (G protein), beta polypeptide 2; G protein, beta-2 subunit; guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 2; signal-transducing guanine nucleotide-binding regulatory protein beta subunit
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	2A8
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Flow-Cyt (1:50-100) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human GNB2
Purification	affinity purified by Protein A
Predicted MW.	37 kDa
Observed MW.	37 kDa
Subcellular Location	N/A
Gene ID	2783
Swiss-Prot	P62879
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with GNB2 monoclonal antibody, unconjugated (SLA2036M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GNB2 Monoclonal Antibody, Unconjugated (SLA2036M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GNB2 Monoclonal Antibody, Unconjugated (SLA2036M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GNB2 Monoclonal Antibody, Unconjugated (SLA2036M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-GNB2 antibody (SLA2036M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

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