Product Name	Recombinant Rabbit Anti-BIRC2 Monoclonal Antibody
Cat No.	SLA1586M
Chinese Name	凋亡抑制因子1重组兔单抗
Alias	API1; HIAP2; Hiap-2; MIHB; RNF48; c-IAP1; cIAP1; Api2; Birc3; C330006D17Rik; HIAP1; IAP1; IAP2; MIAP1; MIAP2; MIHC; cIAP2; mcIAP1; rIAP1; BIRC2_HUMAN; BIRC2; Cellular inhibitor of apoptosis 1 (C-IAP1); IAP homolog B; Inhibitor of apoptosis protein 2 (hIAP-2 | hIAP2); RING finger protein 48; RING-type E3 ubiquitin transferase BIRC2; TNFR2-TRAF-signaling complex protein 2; 2.3.2.27; BIRC2_MOUSE; Inhibitor of apoptosis protein 2 (mIAP2); baculoviral IAP repeat containing 2; baculoviral IAP repeat-containing 2; NFR2-TRAF signalling complex protein; apoptosis inhibitor 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	16H6
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human BIRC2
Purification	affinity purified by Protein A
Predicted MW.	70 kDa
Observed MW.	62 kDa
Gene ID	329
Swiss-Prot	Q13490
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with cIAP1 monoclonal antibody, unconjugated (SLA1586M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with cIAP1 Monoclonal Antibody, Unconjugated(SLA1586M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with cIAP1 Monoclonal Antibody, Unconjugated(SLA1586M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with cIAP1 Monoclonal Antibody, Unconjugated(SLA1586M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with cIAP1 Monoclonal Antibody, Unconjugated(SLA1586M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.