Summary:

Purpose

Our Quick Step Bird Epidermal Growth Factor (EGF) ELISA Kit is to assay EGF levels in Bird serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to EGF. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for EGF is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain EGF and HRP conjugated EGF antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of EGF. You can calculate the concentration of EGF in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0051BI is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.048	0.0505
Blank Well	0pg/ml	0.053	0.0505
S1	180pg/ml	1.941	1.9370
S1	180pg/ml	1.933	1.9370
S2	120pg/ml	1.317	1.3255
S2	120pg/ml	1.334	1.3255
S3	60pg/ml	0.709	0.7200
S3	60pg/ml	0.731	0.7200
S4	30pg/ml	0.373	0.3645
S4	30pg/ml	0.356	0.3645
S5	15pg/ml	0.187	0.1900
S5	15pg/ml	0.193	0.1900