Summary:

Purpose

Our Quick Step Guinea pig Interleukin 1β(IL-1β) ELISA Kit is to assay IL-1β levels in Guinea serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IL-1β . Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IL-1β is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IL-1β and HRP conjugated IL-1β antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-1β . You can calculate the concentration of IL-1β in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0050Gp is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.042	0.0485
Blank Well	0pg/ml	0.055	0.0485
S1	120pg/ml	1.998	1.9855
S1	120pg/ml	1.973	1.9855
S2	80pg/ml	1.318	1.3240
S2	80pg/ml	1.330	1.3240
S3	40pg/ml	0.688	0.6915
S3	40pg/ml	0.695	0.6915
S4	20pg/ml	0.393	0.3875
S4	20pg/ml	0.382	0.3875
S5	10pg/ml	0.201	0.1950
S5	10pg/ml	0.189	0.1950