Summary:

Purpose

Our Quick Step Cattle Androgen ELISA Kit is to assay Androgen levels in Cattle serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antibody specific to Androgen. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antibody. Then a Horseradish Peroxidase (HRP)- conjugated Antibody specific for Androgen is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Androgen and HRP conjugated Androgen Antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Androgen. You can calculate the concentration of Androgen in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0047Ot is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.033	0.0360
Blank Well	0pg/ml	0.039	0.0360
S1	900pg/ml	1.985	1.9810
S1	900pg/ml	1.977	1.9810
S2	600pg/ml	1.363	1.3695
S2	600pg/ml	1.376	1.3695
S3	300pg/ml	0.752	0.7480
S3	300pg/ml	0.744	0.7480
S4	150pg/ml	0.384	0.3805
S4	150pg/ml	0.377	0.3805
S5	75pg/ml	0.169	0.1780
S5	75pg/ml	0.187	0.1780