Summary:

Purpose

Our Quick Step Horse Glucose Regulated Protein 78,GRP78 ELISA Kit is to assay GRP78 levels in Horse serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to GRP78. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for GRP78 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain GRP78 and HRP conjugated GRP78 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of GRP78. You can calculate the concentration of GRP78 in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0046Ho is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.052	0.0460
Blank Well	0pg/ml	0.040	0.0460
S1	3600pg/ml	1.912	1.9075
S1	3600pg/ml	1.903	1.9075
S2	2400pg/ml	1.388	1.3840
S2	2400pg/ml	1.380	1.3840
S3	1200pg/ml	0.708	0.7100
S3	1200pg/ml	0.712	0.7100
S4	600pg/ml	0.403	0.4000
S4	600pg/ml	0.397	0.4000
S5	300pg/ml	0.189	0.1960
S5	300pg/ml	0.203	0.1960