Summary:

Purpose

Our Quick Step Plant phytic acid,pc ELISA Kit is to assay pc levels in Plant serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to pc . Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for pc is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain pc and HRP conjugated pc antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of pc . You can calculate the concentration of pc in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0055PI is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/L	0.042	0.0485
Blank Well	0ng/L	0.055	0.0485
S1	120ng/L	1.998	1.9855
S1	120ng/L	1.973	1.9855
S2	80ng/L	1.318	1.3240
S2	80ng/L	1.330	1.3240
S3	40ng/L	0.688	0.6915
S3	40ng/L	0.695	0.6915
S4	20ng/L	0.393	0.3875
S4	20ng/L	0.382	0.3875
S5	10ng/L	0.201	0.1950
S5	10ng/L	0.189	0.1950

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)