DESCRIPTION

PDGFR alpha (Platelet-derived growth factor receptor alpha), also known as CD140a, is a cell surface receptor tyrosine kinase that plays a crucial role in various biological processes. PDGFR alpha has been shown to regulate angiogenesis both in normal and pathological conditions, mainly stimulating the production of vascular endothelial growth factor (VEGF). Moreover, PDGFR alpha expression is correlated with aggressive disease and significantly worse patient survival in pediatric high-grade gliomas and hepatocellular carcinoma.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	2,646.7	23.4	0.9
2	8	680.4	7.0	1.0
3	8	370.6	4.8	1.3

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	2,634.1	54.4	2.1
2	16	690.4	14.5	2.1
3	16	370.5	5.0	1.3

RECOVERY

The recovery of human PDGFR alpha/CD140a spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:16	113	110-114
Lysate	1:16	98	94-104

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The lysate was initially diluted 1:8.)

		Human plasma	Lysate
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	85	101
	Range (%)	85-86	97-105
1:8	Average% of Expected	82	103
	Range (%)	80-84	95-112
1:16	Average% of Expected	79	97
	Range (%)	74-83	79-115

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human PDGFR alpha/CD140a One-Step ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)