DESCRIPTION

PDGFR beta(platelet-derived growth factor receptor beta) is a typical transmembrane tyrosine kinase receptor that promotes blood vessel formation when activated by platelet-derived growth factor (PDGF) and controls many important cellular processes such as growth, proliferation, movement, and survival. PDGFR beta is expressed in neurons, vascular smooth muscle cells, and pericytes. PDGFR beta may serve as a prognostic factor in GC (Gastric cancer) by immunohistochemical expression. In addition, PDGFR beta activation is sufficient to promote cardiomyocyte proliferation and heart regeneration by stimulating EZH2-mediated Ink4a/Arf repression.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	2,521.7	50.0	2.0
2	8	674.4	15.6	2.3
3	8	187.0	4.7	2.5

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	2,516.3	41.4	1.6
2	16	666.5	15.3	2.3
3	16	184.3	11.6	6.3

RECOVERY

The recovery of human PDGFR beta spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:64	94	80-113
Tissue lysate	1:128	89	80-95

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human plasma was initially diluted 1:4. The tissue lysate was initially diluted 1:16.)

		Human plasma	Tissue lysate
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	113	108
	Range (%)	103-123	104-113
1:8	Average% of Expected	114	95
	Range (%)	112-116	87-103
1:16	Average% of Expected	121	95
	Range (%)	116-126	80-111

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human PDGFR beta produced at Sunlong Medical™.