Product Name	Recombinant Rabbit Anti-ATG4C Monoclonal Antibody
Cat No.	SLA1585M
Chinese Name	自噬相关蛋白4C重组兔单抗
Alias	APG4-C; APG4C; AUTL1; AUTL3; HsAPG4C; Atg4cl; ATG4C_HUMAN; ATG4C; AUT-like 3 cysteine endopeptidase; Autophagy-related cysteine endopeptidase 3 (Autophagin-3); Autophagy-related protein 4 homolog C (HsAPG4C); 3.4.22.-; ATG4C_MOUSE; Autophagy-related protein 4 homolog C; autophagy related 4C cysteine peptidase; AUT (S. cerevisiae)-like 1, cysteine endopeptidase; AUT-like 1, cysteine endopeptidase (S. cerevisiae); APG4 autophagy 4 homolog C (S. cerevisiae); ATG4 autophagy related 4 homolog C (S. cerevisiae); autophagy related 4C, cysteine peptidase
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	18B10
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human ATG4C
Purification	affinity purified by Protein A
Predicted MW.	52 kDa
Observed MW.	52 kDa
Gene ID	84938
Swiss-Prot	Q96DT6
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with ATG4C monoclonal antibody, unconjugated (SLA1585M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Testicles ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATG4C Monoclonal Antibody, Unconjugated(SLA1585M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATG4C Monoclonal Antibody, Unconjugated(SLA1585M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATG4C Monoclonal Antibody, Unconjugated(SLA1585M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (ATG4C) monoclonal Antibody, unconjugated (SLA1585M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.