Product Name	Recombinant Rabbit Anti-IVL Monoclonal Antibody
Cat No.	SLA1560M
Chinese Name	囊包蛋白/内披蛋白重组兔单抗
Alias	INVO_HUMAN; IVL; INVO_MOUSE; INVO_RAT; involucrin
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	10A18
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Involucrin
Purification	affinity purified by Protein A
Predicted MW.	68 kDa
Observed MW.	110 kDa
Subcellular Location	Cytoplasm
Gene ID	3713
Swiss-Prot	P07476
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Involucrin monoclonal antibody, unconjugated (SLA1560M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Involucrin Monoclonal Antibody, Unconjugated (SLA1560M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Involucrin Monoclonal Antibody, Unconjugated (SLA1560M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Involucrin Monoclonal Antibody, Unconjugated (SLA1560M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Involucrin Monoclonal Antibody, Unconjugated (SLA1560M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.