Aliases:	YWHAG; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Gamma; 14-3-3GAMMA; PPP1R170; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Gamma Polypeptide; Protein Phosphatase 1, Regulatory Subunit 170; Protein Kinase C Inhibitor Protein 1; 14-3-3 Protein Gamma; KCIP-1; 14-3-3 Gamma; 14-3-3γ; 14-3-3G; EIEE56; DEE56
Background:	UniProt Entry: P61981;NCBI Gene Entry: 7532
Application Information	Molecular Weight: Predicted, 28 kDa, observed, 28 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1970 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human 14-3-3 gamma
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of 14-3-3 gamma expression in HepG2 cells using 14-3-3 gamma antibody 1:2,000. Green, isotype control; red, 14-3-3 gamma.

Immunocytochemical staining of HepG2 cells with 14-3-3 gamma antibody 1:1,000. Nuclei were stained blue with DAPI; 14-3-3 gamma was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-14-3-3 gamma antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-14-3-3 gamma antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.