Product Name	Recombinant Rabbit Anti-PRKAR2A Monoclonal Antibody
Cat No.	SLA3072M
Chinese Name	蛋白激酶A调节亚基2α重组兔单抗
Alias	PKR2; PRKAR2; 1110061A24Rik; RII(alpha); KAP2_HUMAN; PRKAR2A; KAP2_MOUSE; KAP2_RAT; protein kinase cAMP-dependent type II regulatory subunit alpha; protein kinase, cAMP-dependent, regulatory, type II, alpha; protein kinase, cAMP-dependent, regulatory subunit type II alpha
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	2F10
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ICC/IF (1:50-200) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human PRKAR2A
Purification	affinity purified by Protein A
Predicted MW.	46 kDa
Observed MW.	46 kDa
Subcellular Location	Cytoplasm
Gene ID	5576
Swiss-Prot	P13861
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PRKAR2A) monoclonal Antibody, unconjugated (SLA3072M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, then stained with Phalloidin/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.	Figure 2. 4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PRKAR2A) monoclonal Antibody, unconjugated (SLA3072M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min . DAPI (blue, C02-04002) was used to stain the cell nuclei.
Figure 3. 4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PRKAR2A) monoclonal Antibody, unconjugated (SLA3072M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, then stained with Phalloidin/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.	Figure 4. 25 ug total protein per lane of various lysates (see on figure) probed with PRKAR2A monoclonal antibody, unconjugated (SLA3072M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PRKAR2A Monoclonal Antibody, Unconjugated (SLA3072M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PRKAR2A Monoclonal Antibody, Unconjugated (SLA3072M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PRKAR2A Monoclonal Antibody, Unconjugated (SLA3072M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. 4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PRKAR2A) monoclonal Antibody, unconjugated (SLA3072M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.