Product Name	Recombinant Rabbit Anti-SERPINH1 Monoclonal Antibody
Cat No.	SLA1436M
Chinese Name	热休克蛋白-47重组兔单抗
Alias	AsTP3; CBP1; CBP2; HSP47; OI10; PIG14; PPROM; RA-A47; SERPINH2; gp46; BERF-1; J6; SERPH_BOVIN; SERPINH1; Collagen-binding protein (Colligin); SERPH_HUMAN; 47 kDa heat shock protein; Arsenic-transactivated protein 3 (AsTP3); Cell proliferation-inducing gene 14 protein; Rheumatoid arthritis-related antigen RA-A47; SERPH_MOUSE; Serine protease inhibitor J6; SERPH_RAT; serpin family H member 1; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1); serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1); collagen binding protein 1; colligin; heat shock protein 47
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	11A7
React Species	Human,Mouse
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Serpin H1
Purification	affinity purified by Protein A
Predicted MW.	46 kDa
Observed MW.	46 kDa
Subcellular Location	Cytoplasm
Gene ID	871
Swiss-Prot	P50454
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with HSP47 monoclonal antibody, unconjugated (SLA1436M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HSP47 Monoclonal Antibody, Unconjugated (SLA1436M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HSP47 Monoclonal Antibody, Unconjugated (SLA1436M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HSP47 Monoclonal Antibody, Unconjugated (SLA1436M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HSP47 Monoclonal Antibody, Unconjugated (SLA1436M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.