Product Name	Recombinant Rabbit Anti-DYSF Monoclonal Antibody
Cat No.	SLA1344M
Chinese Name	Dysferlin蛋白重组兔单抗
Alias	FER1L1; LGMD2B; LGMDR2; MMD1; 2310004N10Rik; D6Pas3; CR201_G0003058; DYSF_BOVIN; DYSF; Dystrophy-associated fer-1-like protein; Fer-1-like protein 1; DYSF_HUMAN; DYSF_MOUSE; dysferlin; limb girdle muscular dystrophy 2B (autosomal recessive); fer-1-like family member 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	12B8
React Species	Human,Mouse
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Dysferlin
Purification	affinity purified by Protein A
Predicted MW.	237 kDa
Observed MW.	250 kDa
Gene ID	8291
Swiss-Prot	O75923
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Key calcium ion sensor involved in the Ca2+-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Dysferlin monoclonal antibody, unconjugated (SLA1344M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Dysferlin Monoclonal Antibody, Unconjugated (SLA1344M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Dysferlin Monoclonal Antibody, Unconjugated (SLA1344M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Dysferlin Monoclonal Antibody, Unconjugated (SLA1344M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Dysferlin Monoclonal Antibody, Unconjugated (SLA1344M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.