Product Name	Recombinant Rabbit Anti-ATG7 Monoclonal Antibody
Cat No.	SLA1315M
Chinese Name	自噬相关蛋白7重组兔单抗
Alias	APG7-LIKE; APG7L; GSA7; SCAR31; 1810013K23Rik; Agp7; Atg7l; Gm21553; ATG7_CHICK; ATG7; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7 (APG7-like); ATG7_HUMAN; Autophagy-related protein 7 (APG7-like | hAGP7); Ubiquitin-activating enzyme E1-like protein; ATG7_MOUSE; Autophagy-related protein 7 (APG7-like | mAGP7); ATG7_RAT; autophagy related 7; APG7 autophagy 7-like (S. cerevisiae); ATG7 autophagy related 7 homolog (S. cerevisiae)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	16D1
React Species	Human
Applications	WB (1:1000-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) ICC/IF (1:50-200) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human ATG7
Purification	affinity purified by Protein A
Predicted MW.	78 kDa
Observed MW.	72 kDa
Gene ID	10533
Swiss-Prot	O95352
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with ATG7 monoclonal antibody, unconjugated (SLA1315M) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ATG7 Monoclonal Antibody, Unconjugated (SLA1315M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ATG7 Monoclonal Antibody, Unconjugated (SLA1315M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. 4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (ATG7) monoclonal Antibody, unconjugated (SLA1315M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Figure 5. ATG7 was immunoprecipitated from HeLa cell lysate with SLA1315M at 1/100 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP conjugated Goat Anti-Rabbit IgG was used as secondary antibody. Lane 1: HeLa cell lysate (Input). Lane 2: SLA1315M IP in HeLa cell lysate. Lane 3: native Rabbit IgG IP in HeLa cell lysate (negative control).

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

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