Product Name	Recombinant Rabbit Anti-NUP98 Monoclonal Antibody
Cat No.	SLA1307M
Chinese Name	核孔复合蛋白Nup98重组兔单抗
Alias	ADIR2; NUP196; NUP96; Nup98-96; 4732457F17; NUP98_HUMAN; NUP98; 3.4.21.-; ADAR2; NUP98_MOUSE; NUP98_RAT; nucleoporin 98 and 96 precursor; nucleoporin 98kDa; nucleoporin 98; Nuclear pore complex protein Nup98-Nup96; nucleoporin 96
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	12E4
React Species	Human,Rat
Applications	IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Flow-Cyt (1:50-100) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human NUP98
Purification	affinity purified by Protein A
Predicted MW.	198 kDa
Observed MW.	188 kDa
Subcellular Location	Nucleus
Gene ID	4928
Swiss-Prot	P52948
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Nup98 and Nup96 are involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NUP98 Monoclonal Antibody, Unconjugated (SLA1307M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NUP98 Monoclonal Antibody, Unconjugated (SLA1307M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NUP98 Monoclonal Antibody, Unconjugated (SLA1307M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NUP98 Monoclonal Antibody, Unconjugated (SLA1307M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. The SH-SY5Y(H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-NUP98 antibody (SLA1307M, 1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.