Product Name	Recombinant Rabbit Anti-FOXP3 Monoclonal Antibody
Cat No.	SLA1080M
Chinese Name	叉头蛋白P3重组兔单抗
Alias	AIID; DIETER; IPEX; JM2; PIDX; XPID; scurfin; sf; RGD1562112; FOXP3_HUMAN; FOXP3; FOXP3_MOUSE; D3ZKI1_RAT;
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	5C7
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:200-800) IHC-F (1:200-800) IF (1:200-800) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human FOXP3
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	47 kDa
Observed MW.	47 kDa
Subcellular Location	Nucleus
Gene ID	50943
Swiss-Prot	Q9BZS1
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with FOXP3 monoclonal antibody, unconjugated (SLA1080M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (SLA1080M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (SLA1080M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (SLA1080M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (SLA1080M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (SLA1080M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (SLA1080M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.