Product Name	Recombinant Rabbit Anti-CASP14 Monoclonal Antibody
Cat No.	SLA1443M
Chinese Name	半胱胺酸蛋白酶蛋白14重组兔单抗
Alias	ARCI12; caspase-14; MICE; mini-ICE; CASPE_HUMAN; CASP14; CASP-14; 3.4.22.-; CASPE_MOUSE; Mini-ICE (MICE); caspase 14; caspase 14, apoptosis-related cysteine protease; caspase 14, apoptosis-related cysteine peptidase; apoptosis-related cysteine protease
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	11C5
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) ICC/IF (1:50-200) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Caspase 14
Purification	affinity purified by Protein A
Predicted MW.	28 kDa
Observed MW.	30 kDa
Subcellular Location	N/A
Gene ID	23581
Swiss-Prot	P31944
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum.Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin.In vitro has a preference for the substrate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Caspase 14 monoclonal antibody, unconjugated (SLA1443M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase 14 Monoclonal Antibody, Unconjugated (SLA1443M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase 14 Monoclonal Antibody, Unconjugated (SLA1443M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase 14 Monoclonal Antibody, Unconjugated (SLA1443M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Caspase 14 antibody (SLA1443M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.