Product Name	Recombinant Rabbit Anti-A2M Monoclonal Antibody
Cat No.	SLA0868M
Chinese Name	alpha 2 Macroglobulin重组兔单抗
Alias	A2MD; CPAMD5; FWP007; S863-7; A2mp; A2MAC1; A2m1; A2maa; A2mb; Mam; A2MG_HUMAN; A2M; Alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; PZP_MOUSE; Pzp; Alpha-2-macroglobulin (Alpha-2-M); A2MG_RAT; alpha-2-macroglobulin
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	1C6
React Species	Human
Applications	WB (1:1000-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human alpha 2 Macroglobulin
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	160 kDa
Observed MW.	180 kDa
20%" class="prolook_0321">Gene ID	2
Swiss-Prot	P01023
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with A2M monoclonal antibody, unconjugated (SLA0868M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with A2M Monoclonal Antibody, Unconjugated(SLA0868M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with A2M Monoclonal Antibody, Unconjugated(SLA0868M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with A2M Monoclonal Antibody, Unconjugated(SLA0868M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Kidney Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with A2M Monoclonal Antibody, Unconjugated(SLA0868M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.