Product Name	Recombinant Rabbit Anti-RB1 Monoclonal Antibody
Cat No.	SLA2935M
Chinese Name	磷酸化成视网膜细胞瘤基因重组兔单抗
Alias	OSRC; PPP1R130; RB; p105-Rb; p110-RB1; pRb; pp110; Rb-1; pp105; RB_HUMAN; RB1; pRb (Rb); RB_MOUSE; RB_RAT; RB transcriptional corepressor 1; osteosarcoma; retinoblastoma 1; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; RB1 | Rb (phospho-S780); p-Rb; phospho-Rb; p-RB1; RB1 | Rb (phospho-Ser780)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	4H4
React Species	Human
Applications	WB (1:1000-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Rb around the phosphorylation site of S780
Purification	affinity purified by Protein A
Predicted MW.	106 kDa
Observed MW.	110 kDa
Subcellular Location	Nucleus
Gene ID	5925
Swiss-Prot	P06400
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle. The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Human Lung squamous cell carcinoma ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (S780) Monoclonal Antibody, Unconjugated (SLA2935M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (S780) Monoclonal Antibody, Unconjugated (SLA2935M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (S780) Monoclonal Antibody, Unconjugated (SLA2935M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (S780) Monoclonal Antibody, Unconjugated (SLA2935M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (S780) Monoclonal Antibody, Unconjugated (SLA2935M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (S780) Monoclonal Antibody, Unconjugated (SLA2935M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.