Product Name	Recombinant Rabbit Anti-EGFR Monoclonal Antibody
Cat No.	SLA2933M
Chinese Name	磷酸化表皮生长因子受体重组兔单抗
Alias	ERBB; ERBB1; ERRP; HER1; NISBD2; NNCIS; PIG61; mENA; 9030024J15Rik; Errb1; Wa5; wa-2; wa2; ErbB-1; EGFR_HUMAN; EGFR; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; 2.7.10.1; EGFR_MOUSE; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog); erythroblastic leukemia viral (v-erb-b) oncogene homolog (avian); erb-b2 receptor tyrosine kinase 1; EGFR | ERBB1/HER1 (phospho-Y1092); p-ERBB1/HER1; phospho-ERBB1/HER1; p-EGFR; EGFR | ERBB1/HER1 (phospho-Tyr1092)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	4B5
React Species	Human,Mouse
Applications	IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human EGFR around the phosphorylation site of Y1092
Purification	affinity purified by Protein A
Predicted MW.	134 kDa
Observed MW.	170 kDa
Subcellular Location	Nucleus
Gene ID	1956
Swiss-Prot	P00533
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-EGFR (Y1092) Monoclonal Antibody, Unconjugated (SLA2933M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-EGFR (Y1092) Monoclonal Antibody, Unconjugated (SLA2933M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-EGFR (Y1092) Monoclonal Antibody, Unconjugated (SLA2933M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-EGFR (Y1092) Monoclonal Antibody, Unconjugated (SLA2933M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. The HeLa (treated with pervanadate (1 mM, 30min)) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-EGFR (Y1092) antibody (SLA2933M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed	Figure 6. 4% Paraformaldehyde-fixed A431 (treated with pervanadate (1 mM, 30min)) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (phospho-EGFR (Y1092)) monoclonal Antibody, unconjugated (SLA2933M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.