Product Name	Recombinant Rabbit Anti-PDHA1 Monoclonal Antibody
Cat No.	SLA2913M
Chinese Name	磷酸化丙酮酸脱氢酶α1重组兔单抗
Alias	E1alpha; PDHA; PDHAD; PDHCE1A; PHE1A; Pdha-1; ODPA_BOVIN; PDHA1; PDHE1-A type I; 1.2.4.1; ODPA_HUMAN; ODPA_MOUSE; ODPA_RAT; pyruvate dehydrogenase E1 subunit alpha 1; pyruvate dehydrogenase (lipoamide) alpha 1; pyruvate dehydrogenase alpha 1; pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHA1 (phospho-S293); p-PDHA1; phospho-PDHA1; PDHA1 (phospho-Ser293)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	8C4
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human PDHA1 around the phosphorylation site of S293
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	40 kDa
Observed MW.	40 kDa
Subcellular Location	Cytoplasm
Gene ID	5160
Swiss-Prot	P08559
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase(E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with phospho-PDHA1 (S293) monoclonal antibody, unconjugated (SLA2913M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-PDHA1 (S293) Monoclonal Antibody, Unconjugated (SLA2913M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-PDHA1 (S293) Monoclonal Antibody, Unconjugated (SLA2913M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-PDHA1 (S293) Monoclonal Antibody, Unconjugated (SLA2913M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-PDHA1 (S293) Monoclonal Antibody, Unconjugated (SLA2913M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-PDHA1 (S293) Monoclonal Antibody, Unconjugated (SLA2913M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.