Product Name	Recombinant Rabbit Anti-PRKCZ Monoclonal Antibody
Cat No.	SLA2970M
Chinese Name	磷酸化蛋白激酶C zeta重组兔单抗
Alias	PKC-ZETA; PKC2; Pkcz; aPKCzeta; nPKC-zeta; zetaPKC; 14-3-3-zetaisoform; r14-3-3; KPCZ_HUMAN; PRKCZ; 2.7.11.13; KPCZ_MOUSE; KPCZ_RAT; protein kinase C zeta; protein kinase C, zeta; PRKCZ | PKC zeta (phospho-T560); p-PKC zeta; phospho-PKC zeta; p-PRKCZ; PRKCZ | PKC zeta (phospho-Thr560)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	1D12
React Species	Human,Mouse,Rat
Applications	WB (1:1000-2000) IHC-P (1:100-200) IHC-F (1:100-200) IF (1:100-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human PKC zeta around the phosphorylation site of T560
Purification	affinity purified by Protein A
Predicted MW.	68 kDa
Observed MW.	68 kDa
Gene ID	5590
Swiss-Prot	Q05513
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-PKC zeta (T560) Monoclonal Antibody, Unconjugated (SLA2970M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-PKC zeta (T560) Monoclonal Antibody, Unconjugated (SLA2970M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-PKC zeta (T560) Monoclonal Antibody, Unconjugated (SLA2970M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-PKC zeta (T560) Monoclonal Antibody, Unconjugated (SLA2970M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-PKC zeta (T560) Monoclonal Antibody, Unconjugated (SLA2970M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-PKC zeta (T560) Monoclonal Antibody, Unconjugated (SLA2970M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

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